Plasmid

Part:BBa_K210014

Designed by: Makoto Kashima   Group: iGEM09_Kyoto   (2009-10-15)


blunt end clonig vector by Stu1

This plasmid makes you possible to PCR orMPR products blunt end ligation and cloning. 1.Digest BBa_K210014 by Stu1 2.ligation 1.product and PCR or MPR product.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2070
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
    Illegal NotI site found at 21
    Illegal NotI site found at 2076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2070
    Illegal suffix found in sequence at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2070
    Illegal XbaI site found at 2085
    Illegal SpeI site found at 14
    Illegal PstI site found at 28
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109


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